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Fig. 3 | Plant Methods

Fig. 3

From: A streamlined guide RNA screening system for genome editing in Sorghum bicolor

Fig. 3

Plasmid-mediated genome editing in sorghum. a Target sequences in SbFT1, SbFT8, SbFT12, and SbTIL1. b Indel frequencies induced by each sgRNA 72 h after transient protoplast transfection. c Evaluation of genome editing at candidate off-target sites in transfected protoplasts. PAM sequences and mismatched nucleotides are shown in blue and orange, respectively. b and c Indel frequencies (mean ± s.e.m.) were obtained from n = 3 independent experiments

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Plant Methods

ISSN: 1746-4811