Fig. 2
From: A streamlined guide RNA screening system for genome editing in Sorghum bicolor
Viability and transfection efficiency of protoplasts from plants cultivated under three different conditions. a Evans blue staining of protoplasts isolated from plants cultured under each condition. Dead cells and debris are stained blue. Scale bars = 50 µm. b The viability of isolated protoplasts was measured by determining the percentage of protoplasts that were not stained with Evans blue solution, using a hemacytometer. Viabilities (mean ± s.e.m.) were calculated from n = 3 independent experiments. c GFP expression in protoplasts that had been isolated from plants cultivated under three different conditions and then transfected with a plasmid expressing GFP fused to a nuclear localization signal. Scale bars = 20 µm. d Transfection efficiencies (mean ± s.e.m.) were measured as the percentage of protoplasts expressing GFP. Efficiencies were obtained from n = 3 independent replicates. b and d Values (mean ± s.e.m.) were obtained from three independent experiments. One-way ANOVA analysis was applied. ****P < 0.0001; ***P < 0.001; **P < 0.01; ns, not significant (P > 0.05)