Fig. 1
From: A streamlined guide RNA screening system for genome editing in Sorghum bicolor
Protoplast isolation from leaves from sorghum plants cultivated under three different conditions. a Ten-day-old seedlings grown under different conditions. Condition 1: 16 h light/8 h dark for 10 days; Condition 2: 16 h light/8 h dark for 7 days and continuous darkness for 3 days; Condition 3: 16 h light/8 h dark for 3 days and continuous darkness for 7 days. Scale bars = 1 cm. b Workflow of the protoplast isolation procedure. Enzyme-treated protoplasts were harvested by sucrose gradient centrifugation. c Weight of leaves, obtained following different cultivation conditions, used for protoplast isolation. d Yield of isolated protoplasts from leaves of plants cultivated under each condition. c and d Values (mean ± s.e.m.) were obtained from three independent experiments. One-way ANOVA analysis was applied. ****P < 0.0001; ***P < 0.001; **P < 0.01; ns, not significant (P > 0.05)