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Fig. 4 | Plant Methods

Fig. 4

From: Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava

Fig. 4

Overexpression of the Xanthomonas axonopodis pv. manihotis XopAO1 gene in cassava using the pCsCMV2-NC vector

(a) Schematic representation of the pCsCMV2-XopAO1 expression vector. (b) Systemic symptoms on cassava plantlets (cultivar SC8) agroinfiltrated with CsCMV2-XopAO1 or CsCMV2-GFP at 20 dpi. (c) Northern blot analysis of the accumulation of viral genomic and subgenomic RNAs in the systemic leaves of plants infected with CsCMV2-XopAO1 or CsCMV2-GFP at 20 dpi using a CsCMV CP-specific RNA probe. Ethidium bromide-stained rRNA is shown in the lower panels. (s) Western blot analysis of CsCMV CP expression in cassava plants infected with CsCMV2-XopAO1 or CsCMV2-GFP using an anti-CP antibody. The Ponceau S staining of the large subunit of Rubisco was used as a loading control

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Plant Methods

ISSN: 1746-4811