Fig. 2

Comparison of GFP expression and fluorescence levels in cassava plants infected with CsCMV-GFP, CsCMV1-GFP, or CsCMV2-GFP

(a) Schematic representation of pCsCMV-GFP, pCsCMV1-GFP, and pCsCMV2-GFP expression vectors. (b) GFP fluorescence in cassava plants infected with CsCMV-GFP, CsCMV1-GFP, or CsCMV2-GFP under ultraviolet light at 20 dpi. (c) Western blot analysis of GFP abundance in cassava plants infected with CsCMV-GFP, CsCMV1-GFP, or CsCMV2-GFP using an anti-GFP antibody at 20 dpi. Ponceau S staining of the large subunit of Rubisco was used as a loading control. (d) RT-PCR analysis of the stability of the inserted GFP gene in CsCMV-GFP, CsCMV1-GFP, or CsCMV2-GFP (L1 to L3: first, second, and third leaves above the inoculated leaves). The empty vectors pCsCMV-NC, pCsCMV1-NC, pCsCMV2-NC, and pCsCMV-CM were used as the controls