Fig. 2
From: Targeted mRNA demethylation in Arabidopsis using plant m6A editor
Design and construction of PMEs inducing targeted demethylation of SPL9, SPL3, FT, WUS, and STMÂ mRNAs. (A) Schematic representation of positions of the m6A sites and regions targeted by crRNAs. The CDS and UTR are represented by black and gray boxes. Yellow bars represent the m6A sites. Red arrow indicates the crRNA. The crRNA was designed to target sequence near the m6A sites. Black arrows indicate the primers for SELECT-qPCR analysis. (B) Schematic illustration of PME-MCS, PME-WS, PME-FSS, PME-WS-H, and PME-FSS-H.