A new and effective method to induce infection of Phyllachora maydis into corn for tar spot studies in controlled environments

Table 4 Temperature and percentage relative humidity for the growth and development of Phyllachora maydis on corn in greenhouse and growth chamber experiments

Experiment	Location	Range	% RH §	Temperature ºC §	Light intensity µmol/m²/s
1	Greenhouse	Minimum	32	22	163
		Mean	45	23	230
		Maximum	62	24	265
	Growth chamber	Minimum	NA	NA	211
		Mean	NA	NA	240
		Maximum	NA	NA	258
2	Greenhouse	Minimum	19	22	137
		Mean	35	23	199
		Maximum	54	25	230
	Growth chamber	Minimum	29	19	131
		Mean	40	20	216
		Maximum	48	20	320
3	Greenhouse	Minimum	30	23	163
		Mean	37	23	273
		Maximum	54	25	352
4	Greenhouse	Minimum	29	20	97
		Mean	45	21	267
		Maximum	54	24	536

Data were collected while using the new inoculation method
§ Values were collected for 12 days based on the average time to first stroma (Table 5)
NA During experiment 1, temperature and % RH were not recorded in the growth chamber due to technical difficulties. However, the growth chamber was set to 19 °C, 85 to 100% RH, and 8-h photoperiod

ISSN: 1746-4811