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Table 3 Temperature and percentage relative humidity for induction of tar spot in a mist chamber

From: A new and effective method to induce infection of Phyllachora maydis into corn for tar spot studies in controlled environments

 

Range

% RH

Temperature ºC

60 min before inoculation †

  
 

Minimum

48

20

 

Mean

90

21

 

Maximum

100

28

For 20 h ‡

   
 

Minimum

100

19

 

Mean

100

20

 

Maximum

100

21

  1. Data were collected in dark (0 µmol/m2/s) while using the new inoculation method
  2. † The humidifier was set to increase % RH to 100% before inoculation
  3. ‡ Once all the inoculated plants were in the mist chamber the humidifier ran cycles of 10 min every 120 min to maintain continuous 100% RH for 20 h
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Plant Methods

ISSN: 1746-4811