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Fig. 2 | Plant Methods

Fig. 2

From: A new and effective method to induce infection of Phyllachora maydis into corn for tar spot studies in controlled environments

Fig. 2

Representation of the induction of tar spot using the new inoculation method from spore collection to stromata development. (A). Stromata of Phyllachora maydis with extruded spores (note orange mass marked with an arrow) before collection. (B). Collection of spores from field-infected plants using the syringe tip attached to the vacuum. (C). Inoculation of corn leaves by application of spore suspension using a brush. (D). Stromata arising from flecked chlorotic lesions 12 dai (see white arrows). (E). Morphology of stromata 20 dai, where the area between the dashed lines is the area shown in (D). (F). Morphology of stromata 18 dai

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