Screening and optimisation of in vitro pollen germination medium for sweetpotato (Ipomoea batatas)

Table 6 Media used for the Calcium nitrate concentration test

Treatment	pH	Sucrose (g/L)	Polyethylene glycol 4000 (PEG4000) (g/L)	Boric acid (mg/L)	Calcium nitrate (mg/L)	Germination rate (%)
Calcium medium 1	6.0	50	50	100	0	7.9 ± 2.4
Calcium medium 2	6.0	50	50	100	100	61.7 ± 5.7*
Calcium medium 3	6.0	50	50	100	200	71.9 ± 4.1*
Calcium medium 4	6.0	50	50	100	300	79.7 ± 4.6*
Calcium medium 5	6.0	50	50	100	400	61.6 ± 9.4*

Germination rate is the mean ± SD of five replicates. * Indicates the first echelon of multiple comparison results. Based on ANOVA, the mean germination rates differed significantly between the media supplemented with and without Calcium nitrate (P < 0.01), while the differences between the four different Calcium nitrate concentrations were not significant

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