Screening and optimisation of in vitro pollen germination medium for sweetpotato (Ipomoea batatas)

Table 5 Media used for the Boric acid concentration test

Treatment	pH	Sucrose (g/L)	Polyethylene glycol 4000 (PEG4000) (g/L)	Boric acid (mg/L)	Calcium nitrate (mg/L)	Germination rate (%)
Boron medium 1	6.0	50	50	0	200	10.0 ± 3.2
Boron medium 2	6.0	50	50	100	200	64.3 ± 5.5*
Boron medium 3	6.0	50	50	200	200	73.8 ± 5.5*
Boron medium 4	6.0	50	50	300	200	63.9 ± 3.7*
Boron medium 5	6.0	50	50	400	200	47.7 ± 5.7*

Germination rate is the mean ± SD of five replicates. * Indicates the first echelon of multiple comparison results. The ANOVA results showed that the difference in pollen germination rate between the media with and without Boric acid was significant (P < 0.01), while the difference between the four media containing Boric acid was not significant

ISSN: 1746-4811