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Table 4 Media used for the Polyethylene glycol 4000 (PEG4000) concentration test

From: Screening and optimisation of in vitro pollen germination medium for sweetpotato (Ipomoea batatas)

Treatment

pH

Sucrose (g/L)

PEG4000 (g/L)

Boric acid (mg/L)

Calcium nitrate (mg/L)

Germination rate (%)

PEG4000 medium 1

6.0

50

0

100

200

46.4 ± 7.8

PEG4000 medium 2

6.0

50

50

100

200

73.5 ± 5.0*

PEG4000 medium 3

6.0

50

100

100

200

62.2 ± 7.8*

PEG4000 medium 4

6.0

50

150

100

200

34.1 ± 12.8

PEG4000 medium 5

6.0

50

200

100

200

12.7 ± 2.1

PEG4000 medium 6

6.0

50

250

100

200

11.8 ± 3.0

PEG4000 medium 7

6.0

50

300

100

200

11.2 ± 2.8

  1. Germination rate is the mean ± SD of five replicates. * Indicates the first echelon of multiple comparison results. The ANOVA results showed that pollen germination rates differed significantly (P < 0.001) between treatments with different PEG concentrations