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Table 2 Media used for the pH test

From: Screening and optimisation of in vitro pollen germination medium for sweetpotato (Ipomoea batatas)

Treatment

pH

Sucrose (g/L)

Polyethylene glycol 4000 (PEG4000) (g/L)

Boric acid (mg/L)

Calcium nitrate (mg/L)

Germination rate (%)

pH medium 1

4.5

50

100

100

200

24.1 ± 10.9

pH medium 2

5.0

50

100

100

200

30.1 ± 6.3

pH medium 3

5.5

50

100

100

200

52.4 ± 3.7*

pH medium 4

6.0

50

100

100

200

66.9 ± 4.7*

pH medium 5

6.5

50

100

100

200

53.6 ± 5.2*

  1. Germination rate is the mean ± SD of five replicates. * Indicates the first echelon of multiple comparison results. ANOVA showed that the germination rate was significantly higher at pH 5.5, 6.0 and 6.5 than at 4.5 and 5.0 (P < 0.001)