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Table 1 Overview of observed interactions between the MADS-domain proteins AP1, SEP3, PI and AP3. (Yeast 2 Hybrid (Y2H), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Liquid chromatography–mass spectrometry (LC–MS)).

From: One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells

 

Y2H

EMSA or IP

BiFC

LC–MS

FRET

Crystal structure

Literature

AP3-PI-SEP3-AG

    

[12, 16]

AP3-PI-SEP3-AP1

    

 

This study

AP3-PI-AP1

  

 

[12], This study

AP3-PI-SEP3

  

 

[12, 18], This study

PI-PI

 

  

 

[34], This study

AP3-PI

 

 

[34, 38, 47], This study

AP3-AP3

 

  

 

[34], This study

SEP3-AP3

 

 

 

[18, 38], This study

SEP3-PI

 

 

 

[18, 38], This study

SEP3-SEP3

 

  

[16, 18, 33], This study

AP1-AP3

 

 

 

[34, 38], This study

AP1-PI

 

 

 

[34, 38], This study

AP1-SEP3

 

 

[12, 18, 38], This study

AP1-AP1

 

  

 

[34], This study