Fig. 9
From: One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells
Interaction analysis between AP1 and AP3 or PI proteins in N. benthamiana leaf cells. A-C: Co-localisation of PI-mV an AP1-mCh in N. benthamiana leaf cells (A PI-mV signal. B AP1-mV signal. C Merged signal). D–F Co-localisation of AP3-mV an AP1-mCh in N. benthamiana leaf cells (D AP3-mV signal. E AP1-mV signal. F Merged signal). MADS-domain proteins fused to the respective FP were expressed in N. benthamiana leaf cells via theUBQ10 promoter (AP3 and PI) or the XVE < < oLexA-35S estradiol inducible system (AP1). Nuclei were imaged 5–6 days after infiltration and expression of AP1 was induced one day prior to image acquisition. Co-expression of AP1 with AP3 or PI did not lead to a change of protein localisation. (Scalebars: A–F 10 µm) G BINDING [%] (grey) and FRET efficiencies [%] (white) for AP3-mV, AP3-mV AP1-mCh, PI-mV and PI-mV AP1-mCh. Analysis was done as described in Fig. 5. Both the AP1/AP3 and the AP1/PI heteromers displayed low average BINDING (16.46% ± 11.17 and 16.08% ± 7.77 respectively) and comparable FRET efficiencies of 43.61% ± 14.18 (AP1/AP3) and 41.54% ± 18.36. Statistical groups were assigned after multiple comparison with Kruskal–Wallis and a Post hoc test using the criterium Fisher’s least significant difference (alpha parameter is 0.05) (Dashed blue line marks the BINDING cut-off of 10%; Number of repetitions are indicated below BINDING values and number of images with BINDING above 10% are indicated below the FRET efficiency values in the bottom of the plot).