Fig. 8
From: One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells
AP3 and PI protein homomerization in N. benthamiana leaf cells. BINDING (grey) and FRET efficiencies (white) for AP3-mV, AP3-mV + PI-mT2, AP3-mV AP3-mCh, AP3-mV AP3-mV-mCh + PI-mT2, PI-mV, PI-mV + AP3-mT2, PI-mV PI-mCh, PI-mV PI-mV-mCh + AP3-mT2. For each analysed nucleus average BINDING and a corresponding average lifetime of mV were fitted. Nucleoli were excluded from FLIM analysis. mT2 did not have an influence on mV lifetime as FRET can only occur from mT2 towards mV but not vice versa. Nuclei with average BINDING below 10% were excluded from FRET efficiency calculation. Statistical groups were assigned after multiple comparison with Kruskal–Wallis and a Post hoc test using the criterium Fisher’s least significant difference (alpha parameter is 0.05) (Dashed blue line marks the BINDING Cut-Off of 10%)