Fig. 6
From: One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells
Interaction between AP1 and SEP3 proteins in N. benthamiana leaf cells. A–A’’ Co-localisation of SEP3-mV an AP1-mCh in N. benthamiana leaf cells (A SEP3-mV signal. A’ AP1-mV signal. A’’ Merged signal). Co-expression did not lead to a change of localisation. (Scalebars: A–A’’: 10 µm) B BINDING [%] (grey) and FRET efficiencies [%] (white) for AP1-mV, AP1-mV SEP3-mCh, SEP3-mV and SEP3-mV SEP3-mCh. Analysis was done as described in Fig. 5. Mean BINDING (28.15% ± 9.7) and FRET Efficiency (38.53% ± 10.53) measured for SEP3 homomers were comparable to the values obtained for AP1 homomers (compare to Fig. 5). Mean BINDING of the AP1/SEP3 heteromer (36.06% ± 10.01) was slightly increased compared to both individual homomers. Mean FRET Efficiency of the heteromers (42.64% ± 6.26) was not significantly different compared to the SEP3 homomers. Statistical groups were assigned after multiple comparison with Kruskal–Wallis and a Post hoc test using the criterium Fisher’s least significant difference (alpha parameter is 0.05) (Dashed blue line marks the BINDING cut-off of 10%; Number of repetitions are indicated below BINDING values and number of images with BINDING above 10% are indicated below the FRET efficiency values in the bottom of the plot)