Fig. 4
From: One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells
BINDING versus FRET efficiencies in FRET and no-FRET samples. BINDING and FRET efficiencies for donor only (AP1-mV), negative control (AP1-mV NLS-mCh) and FRET (AP1-mV AP1-mCh) samples. In samples where molecules don’t undergo FRET, acquired BINDING usually was between − 10 and 10%. We therefore defined this range below 10% as “limit for FRET efficiency calculation”. When BINDING was below 10%, FRET efficiencies were highly variable and accumulated at the limits (10% and 80% FRET efficiency) which defined in the fitting model. Therefore, we excluded FRET efficiencies when BINDING was below 10% and only include them when molecules in a sample undergo FRET (BINDING above 10%)