Fig. 12

Co-expression of AP1-mT2 with GFP reporeter of SEP3, AP3 and PI. A–C Z-stacks of AP1-mT2 (red) SEP3-GFP (green), AP1-mT2 (red) APP3-GFP (green) and AP1-mT2 (red) PI-GFP (green). A’–C’ signal overlap was calculated in FIJI [19] using the Image calculator tool and were displayed with the “fire” color scale (low signal: purple; high signal: yellow). AP1 and SEP3 expression overlaps in few cells of late stage 2 floral buds and most cells of the dome shaped floral meristem in stage 3, stage 4 and stage 5 flowers. Overlaped expression of AP1 with AP3 or PI is first visibale in stage 3 floral buds. In stage 4 flowers AP1 and AP3 or PI proteins show an overlapping expression in a ringformed pattern while in stage 5 flowers, overlapping expression was restricted to petal initiation sites. Numbers indicate floral stage as previously defined (Smyth, Bowman, and Meyerowitz 1990). Scale bars: 50 µm