Fig. 10
From: One pattern analysis (OPA) for the quantitative determination of protein interactions in plant cells
Localisation of co-expressed MADS-box proteins in N. benthamiana leaf cells. A Localisation of the AP3/PI heteromer visualized by BiFC. BiFC did not interfere with AP3/PI translocation to the nucleus or nucleolus. B–D Localisation of SEP3-mCh, NLS-mCh and AP1-mCh respectively. E–E’’: Co-expression of AP3/PI and SEP3 (E Signal from AP3/PI, E’: Signal from SEP3, E’’ Merged signal). SEP3 accumulated at the nucleolus and fully co-localized with AP3/PI. F–F’’ Co-expression of AP3/PI and AP1 (F Signal from AP3/PI, F’ Signal from AP1, F’’ Merged signal). AP1 weakly accumulated at in the presence of AP3/PI. G–G’’ Co-expression of AP3/PI and NLS-mCh (G Signal from AP3/PI, G’ Signal from NLS-mCh, G’’ Merged signal). NLS-mCh localisation did not change in the presence of AP3/PI. H–H’’’ Co-expression of AP3/PI with SEP3 and AP1 (E Signal from AP3/PI, E’ Signal from SEP3, E’’ Signal from AP1 E’’’ Merged signal). Expression of SEP3 with AP3/P and AP1 lead to strong re-localisation of AP1 to the nucleolus and full co-localisation of all four MADS-domain proteins. (Scale bars: 10 µm)