Fig. 8From: Mapping intron retention events contributing to complex traits using splice quantitative trait locusAssociation of IR with miRNA-mediated regulation. a Pie plot showing the distribution of potential miRNA target sites in IR-coupled genes (left) and cis-sQTL genes [2]. b An example of the IR-coupled miRNA mechanism. GhDCL4 (GH_A05G0514) was detected to have a significant cis-sQTL (A05:4805506:4806040:clu_24377). The IGV screenshot [51] shows the total read numbers for each junction among individuals of different haplotypes, red dotted box shows the IR locus. The transcript model (bottom) shows the impact of IR on predicted miRNA targeting of GhDCL4. Blue boxes indicate the coding sequence, and lines indicate introns. Transcripts labeled “IR” are produced from intron retention. The red box indicates the predicted miRNA target site. c Distribution of PSI (left) and gene expression [2] according to haplotype (CC, CT, and TT) of sSNP A05:4938322. Boxes in box plots span from the first to third quartiles, and center lines indicate the second quartile (median). p-values were calculated by two-sided Student’s t-testBack to article page