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Fig. 4 | Plant Methods

Fig. 4

From: VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells

Fig. 4

SM kymography analysis for the colocalization between AtRGS1-YFP and mCherry-AtREM1.3 under different conditions. A–C The typical 3D viewer of AtRGS1-YFP (A), mCherry-AtREM1.3 (B), and merge (C) images at steady state. Bar = 5 s. D–F The typical 3D viewer of AtRGS1-YFP (D), mCherry-AtREM1.3 (E), and merge (F) images with JA treatment. Bar = 5 s. G The magnified images of the blue rectangle marked areas in A–C. Bar = 1 s. H The magnified images of the blue rectangle marked areas in D–F. Bar = 1 s. The 6-day-old transgenic seedlings co-expressing AtRGS1-YFP and mCherry-AtREM1.3 were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h

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Plant Methods

ISSN: 1746-4811