Fig. 3

SM kymography analysis for the dwell time of AtRGS1-YFP and mCherry-AtREM1.3 under different conditions. A–C The tilt angle of D in xy (A), yz (B), or xz (C) slice. D, E The typical 3D viewer of AtRGS1-YFP with (E) or without JA stimulation (D). F The average dwell time of AtRGS1-YFP with or without JA treatment. **P < 0.01. Student’s t-test. Error bars represent the SD. n = 30. G, H The typical 3D viewer of mCherry-AtREM1.3 with (H) or without JA stimulation (G). Bar = 2 s in D, E and G, H. The 6-day-old transgenic seedlings expressing AtRGS1-YFP or mCherry-AtREM1.3 were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h