Fig. 1

Distribution of the AtRGS1-YFP and mCherry-AtREM1.3 protein on the PM under different conditions. A, B Representative single-particle images of AtRGS1-YFP in Arabidopsis hypocotyl cells by VA-TIRFM. The 6-day-old transgenic seedlings expressing AtRGS1-YFP were treated with ½ MS liquid medium (CK) and 100 μM MeJA (JA) for 8 h; C, D representative single-particle images of mCherry-AtREM1.3 in Arabidopsis leaf epidermal cells by VA-TIRFM. The 6-day-old seedlings expressing mCherry-AtREM1.3 were treated with ½ MS liquid medium (CK) or with 100 μM MeJA (JA) for 8 h. Bar = 2 μm in A–D