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Fig. 5 | Plant Methods

Fig. 5

From: A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in C3 and C4 grasses

Fig. 5

Comparison of chloroplast numbers obtained from two and three-dimensional (2D and 3D) imaging. a Representative two-dimensional (left) and three-dimensional (right) images of wild-type rice leaves. Bundle sheath cells are highlighted with white lines. Chloroplasts present in the 3D image but not detected in the 2D image are highlighted with white arrows. b Chloroplast number per bundle sheath cell - each observation represents one cell (n = 31). c Planar chloroplast area of bundle sheath cells - each observation represents one chloroplast. Number of chloroplasts (n) = 236 and 242 from 2D and 3D imaging, respectively. Blue dot in the violin plots represent mean values. Statistical test: t-test. M: Mesophyll tissue

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Plant Methods

ISSN: 1746-4811