Fig. 4

Visualization and quantification of chloroplast area and number in the bundle sheath of rice. a Representative maximum intensity projection image of a Z-stack from wild-type and the GOLDEN2 overexpressing line (pZmUbi::ZmG2). Images were cropped to focus on a bundle sheath strand. Bundle sheath cells are highlighted with white lines. b Planar area of individual chloroplasts from bundle sheath cells. Each observation represents one chloroplast. Number of chloroplasts (n) = 1032 and 1114 from wild-type and pZmUbi::ZmG2 rice lines, respectively. c Chloroplast number per bundle sheath cell. Each observation represents one cell. d Total chloroplast area per bundle sheath cell. Each observation represents the total chloroplast area of a cell. Number of cells (n) = 82 and 90 from wild-type and pZmUbi::ZmG2 rice lines, respectively. Blue dot in the violin plots represent mean values. Statistical test: t-test