Fig. 3

Quantification of bundle sheath cell dimensions in rice and maize. a Representative confocal laser scanning microscopy images of calcofluor white stained (pseudo colour: grey) bundle sheath strand and mesophyll tissue of rice (a) or maize (b). Images were cropped to focus on a bundle sheath strand. Violin plots representing bundle sheath cell cell width (c), length (d) and area (e) in rice and maize. Blue dot represents mean values. Bundle sheath cell length and width measurements were taken at the mid-point of the proximal-distal and medio-lateral axes respectively (annotated with white arrows in a and b). Each observation represents one cell. Number of cells (n) = 82 and 90 from rice and maize, respectively. Abbreviations are as follows: V: Vein; BS: Bundle sheath cell; M: Mesophyll cell. Statistical test: t-test