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Fig. 2 | Plant Methods

Fig. 2

From: A rapid and robust leaf ablation method to visualize bundle sheath cells and chloroplasts in C3 and C4 grasses

Fig. 2

Visualization of rice and maize leaves before and after ablation. Light microscopy images of rice (a–d) and maize (e–h) leaves before (left) and after scraping (right). Low power images (b, f) illustrating the impact of ablation on bundle sheath visibility (highlighted with white lines). Representative images of selected regions at higher magnification (d, h). Bundle sheath cells highlighted with white lines. Abbreviations are as follows: 2°: secondary veins; 3°: tertiary veins; V: Vein; BS: Bundle sheath cell. M: Mesophyll tissue. Scale bar represents 100 μm (a, b, e, f) and 20 μm (c, d, g, h)

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Plant Methods

ISSN: 1746-4811