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Fig. 5 | Plant Methods

Fig. 5

From: Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy

Fig. 5

Deuterium content (%) obtained by CRM for roots and by EA-Cr/HTC-IRMS for roots and leaves. Root zones: Y - young (root tip), M - middle, O - old. Leaf zones: T - tip, C - center, B - base. Tn is the time of harvest in days after exposure to D2O. N refers to non-labeled samples. Deuterium content in roots was shown by blue and red bars measured with CRM and EA-Cr/HTC-IRMS, respectively. With increasing exposure time, the deuterium content in Y roots increased and was higher than for M and O root zones. The deuterium content measured in the roots by both techniques was comparable. Deuterium content in leaves measured by EA-Cr/HTC-IRMS (light red bars at top) increased from day 1 to day 3 and then decreased on day 4. T zones of leaves represented lower deuterium incorporation than C and B zones. Error bars show error in calculations

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