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Fig. 3 | Plant Methods

Fig. 3

From: Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy

Fig. 3

Registered HIM-CRM maps showing the distribution of CD% and lignin% in Y and M roots. The distribution of CD% in YDT2 (A) and MDT2 (B) as well as the lignin% distribution in YDT2 (C) and MDT2 (D) are shown in this figure. The heat maps false color-code the distribution of CD% and area of the lignin band normalized on the sum of the CH and CD areas in the Raman spectra. The rectangles show areas mapped by CRM and then registered onto HIM micrographs using the Correlia plug-in in ImageJ software. The yellow/white area in A & B panels represents the highest CD%. Both roots accumulated more deuterium in the xylem and phloem than in the cortex. The more mature root MDT2 (D) contained much more lignin (yellow color) than the root tip YDT2 (C). The shadow effect in panels A & C is most definitely due to the not perfectly flat surface of the root cross-section. We assume that the electron from the electron flood gun did not reach the surface in that area

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Plant Methods

ISSN: 1746-4811