Fig. 5

Effect of phytohormones on the expression of nLUC-DAO. On day 12, seedlings were subjected to different treatments: a 2-h heat shock at 38 °C, exposure to various phytohormones for 4 h at 31 °C, or no treatment at 22 °C. A Differential expression of the transgene in the presence of DMSO (mock), 50 µM ABA, 50 µM Radicicol, 100 µM salicylic acid (SA), 1 µM Epibrassinolide (EpiBR), 50 µM Radicicol at 22 °C for 2 h followed by heat shock at 38 °C for 2 h, and 2 h at 38 °C and then addition of 50 µM Radicicol for 2 h. B Differential expression of the transgene in the presence of ethanol (EtOH, mock), 40 µM Jasmonic Acid (JA), 100 µM Methyl-Jasmonate (JA), or 40 µM Indole-3-acetic acid (IAA, auxin). Statistical analysis using ordinary one-way ANOVA was performed, and asterisks indicate statistically significant differences (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, Not Significant)