Fig. 3
From: Design of an Arabidopsis thaliana reporter line to detect heat-sensing and signaling mutants
Effect of chemical compounds on the expression of nLUC-DAO1 at different temperatures. A Hydrogen peroxide (H2O2). B Mannitol. C Sodium chloride (NaCl). D Flagellin (FLG22). On day 12, twenty-two seedlings were exposed to either 250 µM H2O2, 300 mM mannitol, 150 mM NaCl, or 1 mM FLG22 for 5 h at 22 °C, followed by 2 h at the indicated temperatures and then 2 h at 22 °C. The absence of H2O2, mannitol, NaCl, and FLG22 is represented by the black bars, while their presence is indicated by the red bars. Statistical significance was determined using the Student's t-test (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, NS, Not Significant). The data are presented as means ± standard deviation (n = 4)