Fig. 1
From: Design of an Arabidopsis thaliana reporter line to detect heat-sensing and signaling mutants
Heat-induced expression of nLUC-DAO bioluminescence. A Two-week-old HIBAT seedlings, grown at 22 °C, were subjected to a 2-h heat treatment at 36 °C and subsequently sprayed with a 1/100 dilution of furimazine. Images were captured after a 2-h recovery period at 22 °C. B Immunoblot analysis was performed to detect sHSP17.7 in Col-0, HIBAT, and a quadruple hsfa1 knockout (KO) mutant. Leaves were exposed to 22 °C, 34 °C, or 38 °C for 2 h, followed by a 2-h recovery at 22 °C. C The expression of the heat-inducible nLUC-DAO was assessed in HIBAT seedlings exposed to different temperatures for 2 h, followed by a 1-h recovery at 22 °C. The concentration of expressed nLUC-DAO proteins was quantified and compared to a calibration curve using purified nLUC. The results are presented as means ± standard deviation (S.D.) with a sample size of n = 8