Fig. 3

In-depth analysis of wrky30 mutant lines by PCR and long-read DNA sequencing. a PCR genotyping of wrky30 mutant lines. Pools were assembled from five randomly selected plants derived from indicated T₃ populations; two pools per population. Corresponding DNAs were used for PCR genotyping (see Fig. 1 for primer binding sites): Amplicon JO244/250 spans the WRKY30 locus; a smaller fragment is amplified in deletion lines. JO248/249 amplify a fragment within WRKY30, absent in deletion lines. JO242/243 amplify a fragment of the zCas9i gene to query presence/absence of the T-DNA. Col-0 and a T₁ individual (1095.14) were included as controls. b Read mappings derived from long-read sequencing (Pacific Biosciences HiFi) of DNA pools (~ 20 plants) derived from indicated populations. Read mappings were visualized using Integrative Genome Viewer [42]. The WRKY30 locus and ~ 1 kb of up-/downstream sequences is shown