Fig. 2
TREX2 improves editing efficiency and alters mutation profiles toward larger deletions. a Overall mutation frequencies at the six target sites with constructs containing or not TREX2. Amplicons containing the target sites up- or downstream of WRKY30 were PCR-amplified using DNA from pooled T1 individuals (transformation of pDGE1081-1090). Up- and downstream amplicons for each construct were pooled and Illumina-sequenced. Mutation frequencies were determined using CRISPResso. Color code (target sites/sgRNAs) as in Fig. 1b. b Guide RNA efficiencies at single target sites. As in (a), but individual target sites ± TREX2 are shown. c Mutation profiles over six target sites upon editing with constructs containing or not the TREX2 exonuclease gene. Samples and data analyses as in (a), but frequencies of different InDels are illustrated. See also Additional file 5: Figure S5 for individual target sites