Fig. 2
From: Fast-track transformation and genome editing in Brachypodium distachyon
Graphic representation of CRISPR/Cas9-generated BdNR mutant alleles. The gRNA target site is represented under each wild-type DNA sequence, “ + ” signs underlining the Cas9 protospacer adjacent motif (PAM). Grey vertical lines indicate the position of predicted Cas9 cleavage sites. Deleted nucleotides are highlighted between parentheses, inserted ones are listed and positioned on top of the corresponding allele. All sequences were deconvoluted with the DECODR algorithm [4] based on Sanger chromatographs generated from both DNA strands, except for plant #101 for which only ( +) strand chromatographs yielded predicted alignment with the wild-type NR1 sequence and plant #303 for which the large NR1 deletion (− 253 nucleotides) was positioned manually