Fig. 6

Diffusion of fluorescein sodium salt through isolated cuticular membranes in the presence of surfactants. The chart represents tracer diffusion (flux) in the presence of surfactants at 1% v/v concentration in the donor chamber over 24 h. The line marker triangle (orange colour) represents amount of tracer diffused through isolated CM without the presence of surfactants. Line marker square (grey colour) represents amount of tracer diffused in the presence of EVO. Line marker diamond (yellow colour) represents amount of tracer diffused in the presence of FAE. Line marker cross (green colour) represents tracer amount of tracer diffused in the presence of OS. Line marker round (blue colour) represents amount of tracer diffused in the presence of AA. The presence of surfactants AA, FAE, and EVO showed increased diffusion of tracer compared to OS and in the absence of surfactants (fresh). The error bar represents standard deviation.