Fig. 2

In-planta gene editing of PeVDE gene in bamboo using CRISPR/Cas9 technology. a Structure of the PeVDE gene and sequences of the target sites. The red triangles represent the forward and reverse primers. b–d NPQ imaging of leaves following infection with Agrobacterium harboring different constructs: 35S::RUBY (b), the mixture of 35S::RUBY and PeVDE CRISPR/Cas9 (c), PeVDE CRISPR/Cas9 (d). The numbers represent the NPQ values of the infected and non-infected areas. e Results of PCR of genomic DNA obtained from wild-type (WT) non-infected and infected leaves using gRNA-2 after 5 or 10 days of infection. + and—represent PCR products with or without AgeI digestion, the different bands are indicated with red arrows. f, g Deep sequencing results of the wide-type (f) and mutated PeVDE clones edited using gRNA-2 (g). Portions of sequences in red, blue, and grey indicate the target sites, PAM, and insertions, respectively. The red dashes indicate deleted nucleotides