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Table 1 Sequencing and SNP-calling data generated from GBS and 3D-GBS libraries of 16 soybean samples

From: 3D-GBS: a universal genotyping-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes

Steps

Measured parameters

GBS

3D-GBS

Sequencing

Mean read count (M)

0.6

0.6

Coverage (%)a

4.7

1.2

Mean depth of coverage (X)b

5.1

14.5

Mean mapping quality

41

42

SNP calling

SNP count

7904

4826

Proportion of missing data (%)

33.7

15.3

Proportion of heterozygous genotypes (%)

4.4

3.8

Average minor allele frequency (%)

33.8

31.3

Nucleotide diversity (p per bp)

0.43

0.42

Physical map

SNP/Mb

8.3

5.1

Number of gaps > 5 Mb

9

6

Number of gaps > 10 Mb

1

0

Genetic map

SNP/cMc

3.7

2.3

Number of gaps > 10 cM

7

10

Number of gaps > 20 cM

0

1

  1. aFraction of the genome captured across all 16 libraries
  2. bAverage number of read at each sequenced position
  3. cInferred from the closest corresponding SNP on the consensus genetic map [16]