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Table 1 LAMP primer sets designed for each target mutation with indication of the strategy used

From: Can allele-specific loop-mediated isothermal amplification be used for rapid detection of target-site herbicide resistance in Lolium spp.?

Gene

Target mutation

Primer sets

Reference tested plants

ALS

197

#1_FIP5′

IT595.4 (−/−) GR20.7 (+ / +)

#2_BIP5′

376

#1_FIP5′

IT533.4 (−/−) IT595.1 (+ / +)

#2_FIP3′

574

#1_FIP3′

IT595.1 (−/−) DK47.6 (+ / +)

#2_BIP3′

#3_FIP5′

#4_BIP5′

ACCase

1781

#1_FIP5′

IT620.10 (−/−) GR9.6 (+ / +)

#2_BIP5′

2041

#1_FIP5′

IT620.10 (−/−) GR20.10 (+ / +)

#2_BIP5′

2078

#1_FIP5′

IT620.10 (−/−) GR20.6 ( ±)

#2_BIP5′

  1. Reference plants used for LAMP primer sets design, as well as tested during protocol set-up phase, and the genotype of target mutations (−/− for WT, + / + for MUT homozygous and ± for MUT heterozygous) are reported for each plant (see Additional file 1 for the chromatograms of the 'Reference tested plants’ in the different target mutations considered for ALS and ACCase gene, respectively)
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Plant Methods

ISSN: 1746-4811