Fig. 4

Example of amplification plots of AS-LAMP reaction on point mutation 376 of ALS gene. Three plants of populations 678 (plants 2, 4 and 5) were analysed with the specific WT primer set (continuous lines) and MUT primer set (dashed lines) for the detection of mutation 376. A negative control (i.e. only water was included in the reaction mix) for both primer sets was also included (pink lines). To facilitate the reading, the lines were coloured in grey when the prediction was correct and therefore consistent with the genotyping, in yellow when they didn’t and in black when the results were not determined (Δt < 5 min)