Fig. 7

Pipeline can quantify petiole growth and margin patterning disruption in jaw-D. A, B Cell areal growth rates for 7–8 DAS displayed on 7 and 8 DAS meshes for WT A or jaw-D B leaf samples. C Quantification of areal growth rates of petiole cells. Jaw-d petioles have higher growth rates on average, with less variability (Student’s t-test p < 0.0001. CV asymptotic test p < 1.578488e−19). D–E Side views of WT (top, white outlines) and jaw-D (bottom, yellow outlines) leaves with margin cells selected in red. Elongated margin cells form a continuous border around the edge of WT leaves, but jaw-D leaves have gaps. F–I Quantification of areal growth F, cell divisions G, cell area H and cell aspect ratio I for all cells 10 µm from margin from 3 to 8 DAS F–G or cells 25 μm from the margin from 5 to 7 DAS H–I. Growth and divisions are largely indistinguishable between cells at the margin between WT and jaw-D. Cells near the WT margin are larger and more elongated than jaw-D, so margin differentiation may be disrupted. (Student’s t-test * = p < 0.05, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001). All measurements are from three replicates for each condition. See Tables 2, 3. for replicate cell counts