Fig. 5
From: Identification of an emerging cucumber virus in Taiwan using Oxford nanopore sequencing technology
Serological assays of the produced antiserum RAs-CBLV to the CBLV-TW coat protein (CP) by indirect ELISA and immunoblotting. a Titration assay. The antiserum was serially diluted for assay. The crude leaf saps of CBLV-TW-infected and healthy (H) Chenopodium quinoa plants at a 1/50 dilution were used to determine the endpoint dilution of RAs-CBLV as 1/640,000. b Sensitivity assay. The quantified purified CBLV-TW CPs were used to react with RAs-CBLV at a 1/5000 dilution. The detection limit of RAs-CBLV was determined as 100 pg CP. c and d Specificity assay. Crude leaf saps of C. quinoa infected with BBWV-2, MYSV, PRSV-W, PVMV, TSWV and ZYMV were incubated with RAs-CBLV in indirect ELISA and immunoblotting, and no signals were detected. Purified CBLV-TW CP and CBLV-TW-infected plant tissue were used as positive controls. The expected 41-kDa CP detected in immunoblotting is indicated by an arrow