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Fig. 4 | Plant Methods

Fig. 4

From: A simple and robust method for isolating and analyzing chromatin-bound RNAs in Arabidopsis

Fig. 4

The overview of CB-RNA-seq and mRNA-seq. A Three examples showed CB-RNA-seq (red) and mRNA-seq (blue) results. A 5ʹ to 3ʹ declining slope is observed in the CB-RNA-seq. The gene structure is indicated at the top of each track, and the length of each gene is indicated at the bottom. B Meta profile showed the reads distribution of CB-RNA-seq and mRNA-seq along the gene. TSS: transcription start site. PAS: Polyadenylation site. C Comparison of gene’s intron/exon ratio between CB-RNA-seq and mRNA-seq. ***p < 0.001, Wilcoxon test. D Diagram showed the calculation method of 5ʹ SS ratio and 3ʹ SS ratio. E Boxplot showed splicing ratio of 5ʹ SS and 3ʹ SS ratio of CB-RNA-seq and mRNA-seq in Arabidopsis. The boxplot showed the median and the 25th and 75th percentiles

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