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Fig. 4 | Plant Methods

Fig. 4

From: IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction

Fig. 4

IMPLANT results in O. sativa with direct PCR. A. Amount of plant material needed for (1) IMPLANT, which is 0.5 × 0.5 mm2 of plant material (magnified in inset), (2) and (3) are upper (500 mg) and lower bounds (33 mg) for generating 10 μg of gDNA with the Qiagen DNeasy Plant Pro Kit. This amount of gDNA suffices for one Southern blot experiment, or for 200 ddPCR reactions. B. IMPLANT direct PCR results. The signal intensity of the competitor is set relative to the intensity of the endogenous sequence (dotted green line). A correction factor of 2 was used for these data. Corr., corrected; Inferr., inferred

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Plant Methods

ISSN: 1746-4811