Fig. 2

IMPLANT results and segregation analysis in A. thaliana. A. Overview of the modified hygromycin resistance marker in pICSL11059, with indication of the competitor (408 bp) and the amplified fragment of the endogenous Arabidopsis gene AT2G28000 (370 bp). B. IMPLANT results using capillary gel electrophoresis. The signal intensity of the competitor is set relative to the intensity of the endogenous sequence (dotted green line), named “Ratio”. The correction factor was empirically determined and set to 2.9 and the corrected ratios (“Corr.”) were rounded up or down to the nearest integer to obtain the inferred copy number (“Inferr.”). The segregating T2 lines that follow a 3:1 segregation ratio in our segregation analysis, but that show the presence of more than one T-DNA copy, are indicated in red. C. Chi square calculation of segregation analysis results. The chi-squared value is calculated for three cases, when there is one transgene-containing locus, leading to three out of four resistant plants, as well for two and three loci. When the calculated value was significant it is printed in black, if not, in gray. The significance of the copy number estimation is denoted by an asterisk (at the 5% significance level). R, resistant; S; sensitive; χ2, Chi square; CN, copy number