Fig. 2

The HIgh Resolution ROot Scanner (HIRROS) setup for automated and non-destructive visualisation of root architecture of seedlings grown in agar plates. Global view of the device (A) with the culture zone on the right side and the imaging zone on the left side. Overview of the imaging zone (B), which is composed of a 16Mpixel linear camera (*), a telecentric objective of a diameter of 25 cm (**), a mobile imaging cart (***) holding plates during imaging, and a collimated LED backlight (****). The square Petri dishes are maintained vertically in metal racks. They are stored in the culture zone (right side of the device). The rack moves to the imaging area (C) for image acquisition, and the plate is pushed in the imaging with a moving arm. Once the image is captured, the plate moves back to its initial location, and the rack moves to image the next plate. Once all plates are imaged, the rack moves back to the culture zone to proceed to the next one. Representative original grayscale image of Arabidopsis seedlings taken by the camera (D). 200 Petri dishes of 12 × 12 cm or 72 Petri dishes of 24 × 24 cm containing up to 1000 Arabidopsis plants fit into HIRROS