High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing

Široká, Jitka; Brunoni, Federica; Pěnčík, Aleš; Mik, Václav; Žukauskaitė, Asta; Strnad, Miroslav; Novák, Ondřej; Floková, Kristýna

doi:10.1186/s13007-022-00954-3

Plant Methods

Table 2 Validation parameters

From: High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing

Analyte	Method accuracy / precision^a,b					RE^b,c, %	PE^b,c,d, %	ME^b,c, %
Spike	0.1 pmol	0.5 pmol	1.0 pmol	5.0 pmol	10.0 pmol
IAA	88.02/5.15	93.55/2.17	105.00/1.83	114.94/1.32	114.04/2.54	105.23 ± 6.53	61.61 ± 7.08	62.21 ± 3.14
oxIAA	94.16/9.37	106.24/1.08	113.01/2.06	113.97/2.66	113.74/1.21	32.11 ± 4.85	39.38 ± 9.66	98.02 ± 0.97
IAA-Asp	90.85/6.00	97.81/4.11	102.30/3.75	112.48/2.82	110.10/2.20	40.02 ± 2.63	46.25 ± 14.26	90.82 ± 1.34
IAA-Glu	92.73/0.70	109.57/4.04	112.09/1.71	113.40/2.40	112.48/2.47	66.38 ± 5.49	75.65 ± 14.20	111.86 ± 2.38
IAA-glc	NC	105.37/9.55	100.37/1.74	105.25/14.48	106.74/11.55	52.11 ± 7.83	37.71 ± 7.89	64.11 ± 4.83
oxIAA-glc	103.95/5.91	110.18/1.78	89.80/2.87	92.13/0.48	92.50/2.23	21.87 ± 8.24	28.25 ± 12.74	114.27 ± 3.34
cis-OPDA	94.92/0.87	102.94/1.72	114.65/2.57	112.29/2.88	113.96/1.73	42.71 ± 15.20	55.29 ± 15.00	102.80 ± 16.40
OPC-8	NC	NC	72.38/7.51	64.15/11.98	62.94/11.8	23.33 ± 1.30	–	112.27 ± 9.56
OPC-6	NC	89.02/7.16	86.27/3.67	90.84/6.03	87.64/4.25	71.02 ± 14.11	–	99.59 ± 8.32
OPC-4	111.04/5.83	97.33/2.63	93.69/7.36	103.65/2.7	100.87/0.58	103.03 ± 6.78	–	77.40 ± 4.28
dn-OPDA	101.73/7.03	105.74/4.96	108.58/3.32	103.68/4.26	97.80/4.47	86.43 ± 7.96	–	76.40 ± 0.82
JA	89.30/1.38	108.26/1.87	111.87/0.92	114.77/3.25	113.74/2.09	99.63 ± 2.60	70.73 ± 11.17	79.13 ± 1.21
MeJA	85.63/4.50	100.14/2.40	111.67/2.21	114.85/0.84	114.40/1.54	79.32 ± 5.84	40.40 ± 10.69	39.56 ± 7.87
9,10-dhJA	86.61/2.54	97.66/3.57	102.26/1.93	114.96/1.82	114.81/0.83	101.32 ± 2.33	–	81.84 ± 3.36
11-/12-OHJA	112.15/9.14	87.40/5.35	85.25/1.46	85.07/4.43	85.18/2.74	54.65 ± 7.25	–	70.76 ± 3.70
JA-Val	90.66/4.48	85.38/1.03	93.01/1.01	89.40/4.77	85.66/4.77	101.71 ± 5.96	–	100.09 ± 1.21
Ja-Ile	85.28/2.75	94.01/3.96	109.18/0.63	114.86/2.07	113.61/3.25	92.39 ± 14.39	106.74 ± 10.44	111.27 ± 1.01
JA-Trp	NC	114.94/6.34	103.75/4.66	114.06/3.99	112.02/3.34	90.55 ± 7.99	–	103.73 ± 0.90
JA-Phe	99.06/2.83	89.18/3.08	105.49/3.90	112.06/2.98	111.16/4.16	87.20 ± 14.06	–	106.15 ± 1.81
SA	111.82/5.7	85.06/3.55	89.71/2.95	110.52/1.24	110.79/1.01	86.12 ± 6.98	62.84 ± 4.10	62.73 ± 2.57
ABA	85.89/1.73	114.81/2.25	114.98/2.19	112.09/3.47	113.61/2.05	83.58 ± 2.41	77.69 ± 12.72	81.49 ± 1.42
PA	99.81/4.01	112.12/6.21	114.6/0.92	112.97/1.55	109.87/2.96	79.54 ± 1.98	62.77 ± 27.34	86.47 ± 1.19
DPA	NC	103.70/6.61	93.87/3.85	100.43/2.05	106.91/1.44	33.70 ± 5.31	42.65 ± 9.28	110.19 ± 2.37
neoPA	90.64/1.55	108.63/1.72	111.65/2.31	112.84/1.86	114.97/0.20	79.65 ± 3.86	74.24 ± 2.84	99.67 ± 2.05
7′-OHABA	88.33/5.83	105.69/3.28	106.62/3.12	114.27/3.72	110.59/3.10	83.88 ± 3.91	86.43 ± 3.27	108.82 ± 2.25

NC not calculated
^aestimated by spiking 2 mg FW of 10-day-old Arabidopsis thaliana seedlings with authentic standards at four concentration levels
^bvalues are means ± SD (n = 4)
^cestimated by spiking 2 mg FW of 10-day-old Arabidopsis thaliana seedlings with authentic or internal standards at a single concentration level
^dassessed for internal standards labelled with stable isotopes

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