Fig. 5

Uncaging CBA in specific regions within root tissues of the cytokinin-responsive TCSn::GFP reporter line. A and B Transmitted light and reflected GFP fluorescence of root tissue before uncaging. C and D Transmitted light and reflected GFP fluorescence displaying the GFP signal detected after 2 h in the respective irradiated regions (white boxes). E and F Transverse section from z-stack showing highly localised area expressing GFP (orange dotted lines) (as well as background fluorescence produced in the epidermis). C and E illustrate cytokinin responses observed in endodermis, pericycle and vascular tissues, while D and F illustrate cytokinin responses in the endodermis. All images captured using 488 nm tuneable argon laser, while uncaging performed using 720 nm MP laser