Fig. 2

Assessments of Ribo-seq data. A, B Read length distribution of nucleus- (Nucl), mitochondria- (Mt) and plastid- (Pt) encoded transcripts displayed based on read counts (A) or fractions (B). C Genomic features of ribosome footprints grouped by read length. Different genomic features are shown with different colors. Ribosome footprints that mapped to nucleus-encoded genes are presented. D Metaplot of protein-coding transcripts showing strong 3-nt periodicity and high enrichment within expected coding regions. Reads mapped to the three reading frames are shown in red, green and blue. The ribosome footprint position is shown by the first nucleotide of the footprint; thus, the first peak is 12 nt upstream of the start codon, consistent with our previous datasets [3,4,5]